Extracts of garlic rich in allicin and ajoene showed appreciable antimycobacterial activity as compared to standard drugs. To study antibacterial activity of allium sativum, zingiber. Antibacterial activity of gallic allium sativum extracts. Broadspectrum antimicrobial activity, chemical composition. Pdf phytofabrication and characterization of copper. The maximum zone of inhibition was showed against bacillus sp. Originally from central asia, garlic is one of the earliest of.
Antibacterial activity of gallic allium sativum extracts on food borne pathogens 46 annals of microbiology and infectious diseases v1 i1 and bacillus anthrax all of. Garlic allium sativum has traditional dietetic and medicinal uses as an antiinfective agent 1. Antimycobacterial and antibacterial activity of allium sativum bulbs. Antimicrobial activity of essential oil extracts of various onions allium cepa and garlic allium sativum. Garlic extract and allicin have been shown to exert bacteriostatic effects on some vancomy. Cultivars by anh dao thi phan, gabriele netzel, panhchapor chhim, michael e. It is a herbaceous plant with height of 2040 cm, a bulb of strong odour and pungent taste. Antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. Antibacterial activity of garlic extracts at 80 c to 90 c for 5 minutes completely destroyed 28,29. The concentration of the plant extracts was found to be 500mgml. We believe that the article provide support to the antibacterial effects of the essential oil. Garlic oil demonstrated significant antibacterial activity, particularly against methicillinresistant staphylococcus aureus. This was further confirmed when compared with commonly used three commercial antibiotics ciprofloxacin, tetracycline and erythromycin as a positive control. Broadspectrum antimicrobial activity, chemical composition and mechanism of action of garlic allium sativum extracts author links open overlay panel cun chen a chunhong liu b jing cai c wei zhang d weiliang qi a zheng wang e zhibin liu b yi yang b.
Allium vegetables, particularly garlic allium sativum l. Antibacterial activity of carica papaya leaves and allium. Cysteine and glutathione counteract the thiolation activity of allicin, and on addition to the reaction mixture the antibacterial activity is reduced. Garlic allium sativum has been used for a long time as a spice or traditional medicine.
Pdf synergistic antibacterial activity among crude extracts. The results of in silico analysis of allicin compounds showed significant inhibitory activity on the ftsz protein compared to penicillin, with an affinity of 10 kcalmol. Antibacterial activity of garlic allium sativum extract and. Inhibitory effect of allium sativum and zingiber officinale. The antibacterial effect of ginger zingiber officinale and garlic allium sativum extract on escherichia coli and listeria monocytogenes was determined invitro by spectrophotometer method. Turkeychopped garlic tc has antimicrobial activity against 17 bacteria. Garlic extracts were obtained and dilution susceptibility assay was used to determine. Activities bioactive compound mechanism of action references antibacterial allicin chemical interaction with. In this study, antimicrobial activities of two different samples of allium sativum l. The data presented in table 1 shows that garlic aqueous extract exhibited highest antibacterial activity against all tested bacteria except e. Preparaton of standard dilutions of garlic extract the dilution were prepared by using ethylene glycol which. The pharmacological activity of garlic allium sativum and its related compounds.
Pdf antibacterial activity of garlic allium sativum on. Sihombing 1, jansen silalahi 1, dwi suryanto 2 1department of pharmaceutical chemistry, faculty of pharmacy, university of sumatera utara. There was synergistic antibacterial activity among the extracts on s. Umamaheswari and others published antibacterial activity of allium sativum l. I declare that the dissertation antibacterial activity of garlic allium sativum against probiotic bifidobacterium species, which i hereby submit for the degree magister scientiae at the university of pretoria, is my own work and has not previously been submitted by me for a degree at this or any other tertiary institution. Resurgence in the use of natural herbal alternatives has brought. Allium sativum, zingiber officinale, allium cepa, antibacterial activity, kirby bauer. Allicin, the antibacterial principle of allium sativum. The purpose of this study is to determine quantitative antimicrobial activity of garlic against shigella by detecting the mic. The antibacterial activity of extracts and oil was estimated by zone of inhibition method.
In vitro confirmation of the antimicrobial action of. Pdf on jan 1, 2016, poonam prakash and others published phytofabrication and characterization of copper nanoparticles using allium sativum and its antibacterial activity find, read and cite. Grampositive bacteria, including multidrugresistant. Phytochemical characteristics and antimicrobial activity of australian grown garlic allium sativum l. Antibacterial activity of gallic allium sativum extracts on. Tuberculosis is one of the major public health problems faced globally. Antibacterial activity of garlic allium sativum extract and molecular. Antimycobacterial and antibacterial activity of allium sativum. Antibacterial effect of allium sativum cloves and zingiber. Pdf antimicrobial activity of essential oil extracts of. The present study was aimed to evaluate the antibacterial activity of direct crude extracts of carica papaya leaves and allium sativum cloves alone and in combination against multiple drug resistant strains.
Some beliefs suggest that garlic is an excellent natural antimicrobial drug that can be considered as an alternative form of treatment or prophylaxis in gastrointestinal infections. The eberspapyrus mentions external application of garlic for indurations. Pdf antibacterial activity of garlic extract allium. Combined antibacterial activity of stingless bee apis mellipodae honey and garlic allium sativum extracts against standard and clinical pathogenic bacteria. The results showed that zones of inhibition recorded by the isolates depend on the type of bacterial isolates and concentration of the extracts. The antibacterial activityof other and close, chemically, cysteinesulfoxidessmethyllcsandmethylmethane. The cloves of garlic and rhizomes of ginger were extracted. Chemical composition, antibacterial and antioxidant activities of tunisian garlic allium sativum essential oil and ethanol extract raja zouari chekki 1,2,3, ahmed snoussi 1,2, imen hamrouni 1 and nabiha bouzouita 1,2, 1 ecole superieure des industries alimentaires, 58 avenue alain savary, 1003 tunis, tunisia.
Antibacterial activity of garlic allium sativum against. The antibacterial effect of garlic allium sativum and other allium spps has been attributed to s. Pdf antimicrobial activity of garlic extract against. Green biosynthesis of silver nanoparticle using garlic, allium sativum with reference to its antimicrobial activity against the pathogenic strain of bacillus sp. Agar well diffusion method showed that both the tested extracts had antibacterial activity against v. Antibacterial activity of clove extracts syzygium aromaticum l. The antibacterial activity of purple type of allium cepa extract was found to be better as compared to yellow type of allium cepa extract. Garlic allium sativum has traditional dietetic and medicinal uses as an anti infective agent 1. Pdf antibacterial activity of allium sativum, syzygium. Antibacterial activity of aqueous garlic allium sativum. Pdf antibacterial activity of garlic extract allium sativum against. Antimycobacterial and antibacterial activity of allium sativum bulbs v. Asian pacific journal of tropical biomedicine 20, 3 9, 725731. Both the extracts exhibited antibacterial activity against v.
Garlic methanol extract was least effective against all tested bacteria. Pdf in vitro antibacterial activity of clove syzygium. Oct 10, 2016 the antibacterial effect of garlic allium sativum and other allium spps has been attributed to s. The antimicrobial activity shown by garlic extracts in this study agrees with the findings of others,14,2931. All tested bacterial strains were most susceptible to the garlic aqueous extract and showed poor susceptibility to the ginger aqueous extract. Antibacterial activity of garlic allium sativum on staphylococcus aureus and escherichia coli. Antibacterial activity of allium sativum against staphylococcus aureus in vitro. Pdf antimicrobial effects of garlic allium sativum l. Antimicrobialactivityofessentialoilextractsofvariousonions. Antibacterial effect of garlic allium sativum against. Antibacterial effect of garlic allium sativum against clinical isolates of staphylococcus.
Antibacterial activity of garlic oil and allicinrich extract was evaluated against staphylococcus aureus, escherichia coli, bacillus subtilis and mrsa by zone of inhibition method using amoxicillin as a standard drug12. Antimycobacterial and antibacterial activity of allium. Phytochemical characteristics and antimicrobial activity. The effect of these spice extracts at varying concentrations on li is shown in table i. All the plant extracts showed activity against the microbes but the extract from punica granatum were the most versatile. Although garlic has been used for its medicinal properties for thousands of years, investigations into its mode of action are relatively recent. Introduction a member of the liliaceae family, garlic allium sativum is a cultivated food highly regarded throughout the world. Allium sativum, essential oil, antibacterial activity. Our finding shows the fact that the essential oil of a. The minimum inhibitory concentration mic of different bacterial species varied from 0. In vitro antibacterial activity and stability of garlic. The present study describes the synergistic antibacterial activity among crude acetone extracts of a.
In vitro confirmation of the antimicrobial action of fresh and freezedried garlic extracts against human pathogenic bacteria 2, fungi 3, and viruses 4 supports these applications. In addition, allicin compounds contained in garlic. Phytochemical screening and antibacterial and antifungal. Our study compares the antibacterial activity of ethanol extracts of garlic allium sativum, clove syzygium aromaticum, and cinnamon cinnamomum zeylanicum against escherichia coli, vibrio. Phadatare and alka mukne department of pharmacognosy, bombay college of pharmacy, kalina, santacruz e, mumbai400 098, india viswanathan, et al antimicrobial activity of allium sativum bulbs. Comparison of antimicrobial activity of allium sativum cloves from. From the published research articles it is clear that the raw juice of garlic was effective against many. Sulphur compounds in garlic are responsible both for its strong smell, and for its medicinal properties.
In vitro antibacterial efficacy of essential oil of allium. Chemical composition, antibacterial and antioxidant. The ginger rhizomes ethanolic extracts demonstrated antibacterial activity against five clinical isolates with zone of growth inhibition ranging from 4 mm to 16 mm. Antibacterial activity of aqueous extract the antibacterial activity of aqueous allium sativum extract is presented in table 3. This study was based on screening antibacterial activity of green tea along with zingiberofficinale and allium sativum combinationagainst gram positive and gram. Apr 27, 2012 the antibacterial activity was determined by disc diffusion method. Garlic is a hardy perennial member of onion family. Pdf antimicrobial properties of allium sativum garlic. Many studies have reported that the inhibitory action of allium sativum juice on fungi may be due to the formation of hydrogen bonds between the hydroxyl group of phenolic compounds and the active sites of the target enzymes yin and tsao et al. Allium sativum a introduction garlic allium sativum l. Pdf antimicrobial activity of allium sativum against human.
This study focuses the significant antibacterial activity of garlic allium sativum linn. Pdf on apr 5, 2014, atheer khashan and others published antibacterial activity of garlic extract allium sativum against staphylococcus. The antibacterial activity was initially determined by the agar well diffusion method followed by determination of minimum inhibitory concentration mic and the synergistic effect among the two. Hippocrates prescribed eating garlic as treatment for uterine tumors, and dioscorides as official physician of the roman. Synergistic antibacterial activity among crude extracts of. The results of this study indicate that garlic extract allium sativum shows antibacterial potential in vitro. The study was performed in triplicate and the average of 3 readings was considered. Antimicrobial activity of essential oil extracts of various. Antibacterial activity of extracts of allium sativum garlic. This antibacterial activitywas enhanced and highest when garlic extract was heated for 45min at 121 c. Green biosynthesis of silver nanoparticle using garlic.
Antibacterial activity of aqueous garlic allium sativum extracts and virgin coconut oil and their combination against bacillus cereus atcc 14579 and escherichia coli atcc 8939 ninda t. Comparison of antimicrobial activity of allium sativum cloves. Antibacterial activity of extracts of allium sativum garlic, myristica fragrans nutmeg, zingiber officinale ginger, allium cepa onion and piper nigrum pepper has been evaluated against. Effect of vermicompost on yield, quality, and antibacterial. To evaluate the antibacterial properties of allium sativum garlic cloves and zingiber officinale ginger rhizomes against multidrug resistant clinical pathogens causing nosocomial infection.